Application of Silica Nanoparticles in Combination with Bacillus velezensis and Bacillus thuringiensis for Anthracnose Disease Control in Shallot.

<b>Background and Objective:</b> Anthracnose in shallot contributes to significant losses. To solve this issue, silica nanoparticles, in combination with <i>Bacillus velezensis</i> and <i>Bacillus thuringiensis</i> were used together. <b>Materials and Methods:</b> <i>In vitro</i> antagonistic test of <i>Bacillus velezensis</i> B-27 with <i>Colletotrichum gloeosporioides</i> was carried out using dual culture and co-culture methods. Treatment in greenhouse experiments was carried out using single application of silica, <i>B. thuringiensis</i>, <i>B. velezensis</i>, a combination of <i>B. thuringiensis</i> and <i>B. velezensis</i> and a combination of <i>B. thuringiensis</i>, <i>B. velezensis</i> and silica. Detection of <i>B. velezensis</i> in the roots of shallot plants was carried out by PCR using a pair of specific primers. <b>Results:</b> <i>Bacillus velezensis</i> was able to inhibit the growth of <i>C. gloeosporioides</i> mycelium <i>in vitro</i>, both in the dual culture and co-culture methods, by 62.8 and 77.17%, respectively. Treatment of <i>B. thuringiensis</i> and <i>B. velezensis</i>, either individually or in combination with silica, could reduce the intensity of anthracnose disease by 20% each and stimulate the growth of shallot plants. The PCR detection using specific primers on the roots of shallot plants showed that <i>B. velezensis</i> was detected with a DNA band length of ±576 bp. <b>Conclusion:</b> <i>Bacillus velezensis</i> can inhibit the growth of <i>C. gloeosporioides</i> mycelium <i>in vitro</i>. Applying <i>B. velezensis</i>, <i>B. thuringiensis</i> and silica can reduce the intensity of anthracnose disease, promote plant growth and increase plant productivity. Furthermore, <i>B. velezensis</i> was detected in the roots of shallot plants, revealing that the bacteria are well-established.

Early Detection of Fusarium Basal Rot Infection in Onions and Shallots Based on VOC Profiles Analysis.

Gas chromatography ion-mobility spectrometry (GC-IMS) technology is drawing increasing attention due to its high sensitivity, low drift, and capability for the identification of compounds. The noninvasive detection of plant pests and pathogens is an application area well suited to this technology. In this work, we employed GC-IMS technology for early detection of Fusarium basal rot in brown onion, red onion, and shallot bulbs and for tracking disease progression during storage. The volatile profiles of the infected and healthy control bulbs were characterized using GC-IMS and gas chromatography-time-of-flight mass spectrometry (GC-TOF-MS). GC-IMS data combined with principal component analysis and supervised methods provided discrimination between infected and healthy control bulbs as early as 1 day after incubation with the pathogen, classification regarding the proportion of infected to healthy bulbs in a sample, and prediction of the infection's duration with an average R2 = 0.92. Furthermore, GC-TOF-MS revealed several compounds, mostly sulfides and disulfides, that could be uniquely related to Fusarium basal rot infection.

Increasing the productivity of shallot planting and its impact on the agricultural market, West Java.

The main objective of this study is to increase the productivity of shallot planting and its impact on the agricultural market. This study analyzes the transmission of shallot prices at the Caringin Main Market (wholesale) level with shallot prices at the farmer (producer) level using quantitative analysis of the Asymmetric Error Correction Model (AECM). The results showed that long-term transmission with positive ECT coefficients and negative ECT showed the same and significant positive values. Positive ECT is 0.286909 and negative ECT is 0.289266, where when there is a price deviation above and below the average balance line of onion prices at the farmer level, it takes three weeks to adjust to the balance of changes in shallot prices. The onion market at the wholesale or parent market occurs asymmetric price transmission from the wholesale price level (Caringin Main Market) to production prices (at the farmer level). A significant t-value of one percent evidences this. Asymmetric price transmission can be caused by abuse of market power.

The potential of shallot skin powder and actinomycetes metabolites as antimicrobe and antibiofilm in the treatment of eel (Anguilla bicolor bicolor) infected with Aeromonas hydrophila.

BACKGROUND: Eel (Anguilla bicolor bicolor) is an Indonesian export commodity. However, it is facing a problem related to Aeromonas hydrophila, which can cause motile aeromonas septicemia (MAS) and produce biofilm formation. Problem with antibiotic resistance challenges the need of an alternative treatment. Therefore, it is important to explore a solution to treat infection and the biofilm formed by A. hydrophila. OBJECTIVES: In this study, we used shallot skin powder and actinomycetes metabolite 20 PM as antimicrobe and antibiofilm to treated eels infected with A. hydrophila. RESULTS: Shallot skin powder (6.25 g 100 g-1 feed) and Actinomycetes 20 PM metabolite (2 mL 100 g-1 feed) were found to be effective as antimicrobe and antibiofilm agent in treating eels infected with A. hydrophila. Eel treated with antibiotic, shallot skin powder, and actinomycetes metabolite had 80%, 66%, and 73% survival rates, respectively. Other indicators such as red blood cell count, hemoglobin, and hematocrit were increased, but white blood cell count and phagocytic activity were dropped. Biofilm destruction were analyzed using scanning electron microscopy to determined antibiofilm activity of actinomycetes metabolite against biofilm of A. Hydrophila. CONCLUSIONS: Shallot skin powder and actinomycetes metabolite were potential to treat infection of A. hydrophila in eel as an alternative treatment to antibiotics.

Development of effluent-free dry-peeling method for shallots (Allium cepa L. aggregatum): Spectral characterization of mid and long infrared peak wavelengths for maximizing the peeling performance.

Different wavelength emitting infrared (IR) lamps (transparent quartz tungsten (TQT), ruby-coated quartz tungsten (RCQT), and ceramic) were used for dry peeling and evaluating the spectral characteristics of emitted radiations. The maximum temperatures for ceramic, RCQT, and TQT were 560, 662, and 861°C, respectively. The peak wavelength determined by Wien's law was between 3.37 and 21.47 µm. Comparatively, longer wavelength was emitted by ceramic lamp. The spectral emissive power determined by Stefan Boltzmann's law was 1.14-37.49 kW m-2 using the IR emitters at different power levels. The radiant efficiency was higher for the ceramic lamp. The peak wavelength and emissive power had a major influence on the peeling performance and quality during IR peeling. The optimized parameters for IR dry peeling of shallots are 60 mm distance between lamp and product, 59.74% IR power level, 15 min of heating time using the ceramic (peak wavelength 8.16 µm) lamp. Practical Application: Infrared dry peeling of shallots is a sustainable alternative to traditional peeling methods that leaves adverse environmental footprints. Spectral characteristics of the infrared lamp can be used to determine the suitable emitter for the dry-peeling operation. It is necessary to check the wavelength emitted by the source for designing the IR system based on the food product and application. This study will be helpful in food processing industries to use an effective infrared lamp that can efficiently peel the agricultural product and sustainably maintain quality.

Quercetin extraction from small onion skin (Allium cepa L. var. aggregatum Don.) and its antioxidant activity.

In the present study, the maximum yield of quercetin was optimized for the ethanol extraction of small onions (Allium cepa L. var. aggregatum Don.), and the antioxidant activity was investigated in vitro. The extraction of quercetin from the small onion skin was carried out through ethanol solvent extraction with different ratios of ethanol and water. Ethanol: water ratio produced the highest quercetin from the onion skin. RP-HPLC analysis of the extracted material showed 2, 122 mg/g of quercetin and 0.34 mg/g of isorhamnetin. A total of 301.03 mg GAE/g dry weight and 156 mg/g quercetin equivalents were found in the onion skin extract. DPPH and ABTS free radical scavenging potentials of the tested extract (90:10 v/v) were dose-dependent, with IC50 values of 62.27 µg/mL and 53.65 µg/mL, respectively. Therefore, the present study reports that small onion skin extract rich in quercetin may serve as a promising antioxidant and anticancer agent.

Screening of Anti-carcinogenic Properties of Phytocompounds from Allium ascalonicum for Treating Breast Cancer Through In Silico and In Vitro Approaches.

Plants, rich in phytocompounds, have been in usage since time immemorial for treating various diseases, namely, cancer. One such plant species, Allium ascalonicum (Shallot) belonging to Amaryllidaceae family is being studied here for its anti-carcinogenic properties against breast cancer. GC-MS characterization of A. ascalonicum exhibited 48 phytocompounds containing five peak phytocompounds and 13 phytocompounds with anti-carcinogenic properties. These 13 anti-carcinogenic phytocompounds were docked with three hormonal receptors involved in breast cancer malignancy, namely, ERα, PR, and human EGFR with tamoxifen as standard for in silico analysis. The results exhibited three phytocompounds that had better binding scores compared to that of the standard drug, tamoxifen. Lyophilized powder of aqueous A. ascalonicum extract, also referred as ASE, was used for in vitro approaches. Antioxidant study using DPPH assay revealed that the highest percentage of FRSA in ASE, nearly 51%, was observed at 50 µg/ml concentration. Cytotoxicity study on MCF-7 cell line using MTT assay demonstrated IC50 value at 1400 µg/ml and anti-proliferative study using Trypan blue assay for the determination of percentage viability of MCF-7 cells at IC50 concentration was observed to be 49%. Anti-mitotic activity using Vigna radiata seed germination assay revealed clear morphological differences in a dose-dependent manner between the seeds grown at various concentrations of ASE with nearly 56.5% growth inhibition observed at 1500 µg/ml concentration. Hence, this research work proves that Allium ascalonicum has very good anti-carcinogenic properties and this can be confirmed further through in vivo animal model studies and it can also be formulated as a promising drug to treat breast cancer. GC-MS characterization of Allium ascalonicum demonstrated the presence of five peak compounds and thirteen anti-carcinogenic compounds. The thirteen anti-carcinogenic compounds were docked with three target proteins (in silico analysis) involved in breast cancer malignancy and identified the presence of three potential phytocompounds that can be used for treating breast cancer. In vitro approaches also confirmed the presence of anti-carcinogenic properties such as antioxidative potential, cytotoxic, anti-proliferative, and anti-mitotic effects. Hence, Allium ascalonicum can be taken further to in vivo studies so that it can be formulated to treat breast cancer.

Preliminary Studies on Detection of Fusarium Basal Rot Infection in Onions and Shallots Using Electronic Nose.

The evaluation of crop health status and early disease detection are critical for implementing a fast response to a pathogen attack, managing crop infection, and minimizing the risk of disease spreading. Fusarium oxysporum f. sp. cepae, which causes fusarium basal rot disease, is considered one of the most harmful pathogens of onion and accounts for considerable crop losses annually. In this work, the capability of the PEN 3 electronic nose system to detect onion and shallot bulbs infected with F. oxysporum f. sp. cepae, to track the progression of fungal infection, and to discriminate between the varying proportions of infected onion bulbs was evaluated. To the best of our knowledge, this is a first report on successful application of an electronic nose to detect fungal infections in post-harvest onion and shallot bulbs. Sensor array responses combined with PCA provided a clear discrimination between non-infected and infected onion and shallot bulbs as well as differentiation between samples with varying proportions of infected bulbs. Classification models based on LDA, SVM, and k-NN algorithms successfully differentiate among various rates of infected bulbs in the samples with accuracy up to 96.9%. Therefore, the electronic nose was proved to be a potentially useful tool for rapid, non-destructive monitoring of the post-harvest crops.

Study on the characteristics of gluten/alginate-cellulose/onion waste extracts composite film and its food packaging application.

The study aimed to improve the properties of SA-CMC film by gluten (G) blends and bioactive compounds from onion waste extracts (OWEs) peel (OPE) and stalk (OSE). The applicability of film on the quality of peeled shallot onion during storage was also examined. Water barrier (0.62 g/msPa × 10-14) and tensile strength (11.50 MPa) of G/SA-CMC film improved more than SA-CMC film (1.55 g/msPa × 10-13 and 7.05 MPa). OPE and OSE increase the total phenolic content (43.86 and 38.35 mgGAE/g) and radical scavenging activity (88.74 and 68.30 %) of G/SA-CMC film than control (20.33 mgGAE/g and 39.20 %). Microbial load (logCFU/g) in terms of total bacterial count, yeast and mold count of shallot onion packed in OPE (5.34 and 5.21) and OSE (4.26 and 4.21) film was reduced than control (6.03 and 4.68). Thus, the G/SA-CMC/OWEs film had improved properties than SA-CMC film and can be used to store peeled onion at 4℃ for 21 days.

Shallot-enriched amaranth-based extruded snack influences blood glucose levels, hematological parameters, and carbohydrate degrading enzymes in streptozotocin-induced diabetic rats.

Diabetes mellitus affects different age groups, and it is popularly managed using synthetic drugs; however, recent research efforts focus on dietary intervention especially the use of fiber rich crops to circumvent the effects of drugs. Developed shallot-enriched amaranth-based extruded snacks, namely: GCB, Amaranth: shallot: soy cake (55:25:20) %; TYD, Amaranth: shallot: soy cake (75:14.81:10.19)%; DZU, Amaranth: shallot: soy cake (60.43:25:14.57)%; and JKL, Amaranth: shallot: soy cake (70:25:5)%, were evaluated for proximate composition, total phenol and flavonoid contents. Snacks ability to modulate carbohydrate hydrolyzing enzymes in streptozotocin induced diabetic rats were also investigated. AMT (100% amaranth grain extrudate) and CCS (commercial shallot-enriched snack) served as control. Protein contents of snacks provided up to 25% of daily protein requirements for adult. Snacks exhibited high total phenol and flavonoid contents. Animals fed with snacks showed ameliorative effects on hematological parameters, attenuates elevation of enzyme activities in kidney and liver homogenates, and displayed decreased α-glucosidase/α-amylase activities. PRACTICAL APPLICATIONS: Dietary intervention is capable of circumventing the adverse effects of synthetic drugs in the management of diabetes mellitus. The study established that developed shallot-enriched amaranth-based extruded snacks possess significant ability to hamper the activities of key carbohydrate hydrolyzing enzymes in streptozotocin induced diabetic rats, hence, has potential as a functional nutritious snack to curtail hyperglycemia and prevent diabetic complications.

Antiallergic activities of shallot (Allium ascalonicum L.) and its therapeutic effects in allergic rhinitis.

BACKGROUND: Onion has antiallergic activity but lack of evidence for shallot. OBJECTIVE: To determine whether shallot owns similar antiallergic activity to onion and its therapeutic effects in allergic rhinitis when added to standard treatment. METHODS: In-vitro ß-hexosaminidase inhibitory activities of shallot was compared with onion on RBL-2H3 cells. In clinical study, a randomized, double-blind, placebo-controlled trial was performed. Sixteen AR patients were randomized equally into the controls who received cetirizine 10 mg once daily and placebo capsules for 4 weeks, and the treatment who received 3g of oral shallot per day (equivalent to 1 ½ bulbs) and cetirizine. Visual analog scores of overall symptoms (VAS), total nasal and ocular symptom scores (TNSS and TOSS), nasal airway resistance (NAR), and adverse events were assessed. RESULTS: Shallot extract at 200 µg/mL had an average ß-hexosaminidase inhibition rate of 97% while onion extract had 73%. HPLC chromatograms (λ = 290nm) of both plants showed nearly identical patterns of quercetin compounds, such as quercetin 3,4'-diglucoside, quercetin 4'-glucoside, and quercetin. After 4-week of treatment, 62.5% of patients in shallot group and 37.5% of patients in control group showed improvement of post-treatment VAS. TNSS were significantly reduced in both groups, however no difference between groups (P = 0.18). TOSS were significantly improved only in the shallot group (P = 0.01). Adverse events from shallot were not different from placebo. CONCLUSIONS: Shallot had antiallergic activity and similar quercetin compounds to onion. The shallot oral supplement and cetirizine was shown to improve the overall AR symptoms more than cetirizine alone.

Antibacterial and antioxidant potential of the phenolic extract and its fractions isolated from Allium ascalonicum (onion) peel.

Aqueous methanolic (20:80) crude extract of the outer peel of Allium ascalonicum (onions) was fractionated into anthocyanin (II), acidic (i.e., phenolic acids, Ia), neutral A (i.e., flavanols, Ib) and neutral B (i.e., flavonols, Ic) phenols using C-18 and hydrophilic-lipophilic balanced (HLB) Co-polymer cartridges. The fractions were analysed for total phenolic, total flavonoids, antibacterial activity, antioxidant potential and lipoxygenase (LOX) inhibition. The recovery of total phenols and bio functional activity in all fractions were found to be more in HLB. All fractions showed antimicrobial activity against tested strains with phenolic acids (Ia) being most active and flavanol (Ib) least active, while the highest free radical scavenging, reducing power and LOX inhibition was exhibited by flavonol (Ic) and least by flavanols (Ib). HPLC analysis revealed the presence of vanillic acid and quercetin in non-anthocyanin I and cyanidin in anthocyanin II.

The competitiveness analysis of shallot in Indonesia: A Policy Analysis Matrix.

Shallot is a national strategic commodity in Indonesia, but it is development has a fundamental technical, socioeconomic, and policy support problems. Therefore, it is essential to know the competitiveness of shallot in Indonesia and the incentive policy to implement the comparative advantage to become a sustainable competitive advantage. The purposes of this study are to (1) analyze the profitability of shallot farming privately and socially, (2) analyze the competitiveness of shallot farming from a competitive and comparative advantage perspective, (3) review the impact of government policy on shallot farming, and (4) formulate incentive policies in the development of shallot commodities. The empirical results of the Policy Analysis Matrix revealed that shallot farming in production centers in Indonesia has both competitive and comparative advantages. The highest competitive and comparative advantages were found in the dry season in the upland of Malang district with the coefficient values of PCR (Private Cost Ratio) of 0.268-0.508 and DRCR (Domestic Resource Cost Ratio) of 0.208-0.323. The lowest competitive advantage was found in the lowland of East Lombok district in the dry season with a coefficient value of PCR 0.728-0.844. The lowest comparative advantage in the dry season was found in East Lombok district with a DRCR of 0.448, while in the rainy season, it was found in Wonosobo district with a DRCR of 0.522. These results mean that it is more profitable for Indonesia to increase domestic shallot production than to import. Improving shallot competitiveness can be carried out by implementing advanced technology, agricultural infrastructure, capacity building of farmers' resources, and government incentive policies to increase productivity and competitiveness sustainability.

Development of active packaging film from sodium alginate/carboxymethyl cellulose containing shallot waste extracts for anti-browning of fresh-cut produce.

Owing to growing concerns about making pollution-free sustainable environment by reducing the dumping of agricultural waste and convert it into valuable product is a key to carry out the present study. The ultimate goal of this study is to convert shallot onion wastes (SOWs) into active packaging and evaluating the anti-browning effect due to the SOWs holding rich polyphenols and antioxidants. The active packaging film was fabricated by using sodium alginate (SA) and carboxymethyl cellulose (CMC) along with shallot onion waste extract (SOWEs) such as peel and stalk at 0.2% and 0.5% concentration. The film made with SA/CMC/SOWEs had good physical, mechanical, optical and barrier property, higher phenolic and antioxidant activity compared to control. In addition, the effect of SA/CMC/SOWEs film packaging on anti-browning and quality of fresh-cut apple and potato stored at 4 °C was studied. The results show the SA/CMC/SOWEs film had better effect on controlling browning index in fresh-cut apple and potato over the storage of 12 days and 5 days. This study concludes that the SA/CMC film developed with shallot stalk extract can be used for wrapping of fresh-cut fruits and vegetables. It also prevents browning and maintains the overall quality than control and shallot peel incorporated film.

Shallot skin profilling, computational evaluation of physicochemical properties, ADMET, and molecular docking of its components against P2Y12 receptor.

OBJECTIVES: Medicinal plants are a source of many compounds that are useful in the pharmaceutical field for novel drug development. Polyphenols and the flavonoid group in plants are known to have several activities, such as relieving cardio vascular disease (CVD). The outer skin of the shallot which is disposed of as waste is known to have an antiplatelet activity which was tested in vitro assay. To date, there is no study reported on the ADMET profile and physicochemical properties of the active component of the shallot skins. METHODS: The extraction of shallot skins was conducted by ultrasonic irradiation using ethanol. The phytochemical screenings were carried out by TLC and color reaction. The profiling of its active ingredient was presented by GC-MS, HPLC and spectrophotometry UV-vis. Whereas their physicochemical properties were analyzed by ChemDraw 17.00 program and the ADMET predictions were studied using pkCSM online tool. The MVD program was operated in the docking study on protein P2Y12 (PDB ID 4PXZ). RESULTS: The extract showed the presence of polyphenol, flavonoids, quercetin, natalensine-3,5-dinitrobenzoate; bis[2-(2-fluorophenyl)-6-fluoroquinolin-4-yl]amine, benzo[a]heptalene, N-(trifluoroacetyl) methyl-N-deacethyl-colchicine. The ADMET prediction data displayed that the compounds in the extract have good absorption so that they can be used in the oral and transdermal routes. Some components in the extract have lower MDS than clopidogrel. CONCLUSIONS: The ultrasonicated shallot skin extract can be used as additional resources of the active pharmaceutical ingredients and to have the potency to be developed as an oral or transdermal preparation.

Investigation of shallot production system in terms of energy-economic-environmental in Iran.

Assessment of environmental consequences of agri-food products during their life cycle is currently identified as the most important and efficient way to investigate agricultural systems. In addition to the environmental impacts, energy and economic issues are considered major issues in the life cycle of products. The present study aimed to investigate and assess the energy flow, environmental, and economic dimensions during shallot production in farms of Iran. Given the limited number of shallot farmers, the required data were collected from 22 shallot farms through the census method in the Shahrekord region. Based on the results obtained from the energy analysis, energy input and output and energy ratio (ER) were obtained as 107,145 and 36,243 MJ ha-1 and 0.4, respectively. Also, electricity was identified as major contributor to energy consumption with the contribution of 74%. Results related to the economic analysis of shallot production revealed that the values of total production, economic productivity, and benefit-cost ratio (BCR) are 15,672 US$ ha-1, 10.89 kg US$-1, and 8.45, respectively. Based on the life cycle assessment results, the contribution of input consumption in the farms and input production to total environmental impacts were determined as 55 and 45%, respectively. Also, normalization of results showed that the marine ecotoxicity (ME) and global warming potential (GWP) impact categories were the main environmental impacts during shallot production. ME and GWP impacts can be attributed to the indirect emissions of electricity and direct emissions of inputs in the farm, respectively.

Triterpenoid glycosides from the rhizomes of Allium ascalonicum and their anoctamin-1 inhibitory activity.

Ten triterpenoid glycosides including two undescribed compounds (1 and 2) were isolated from the methanol extract of Allium ascalonicum rhizomes. These compounds were structurally elucidated to be 3ß-O-α-L-rhamnopyranosyl-(1→2)-α-L-arabinopyranosyl-19α-hydroxyolean-12-ene-28-oic acid 28-O-[α-L-rhamnopyranosyl-(1→2)-ß-D-glucopyranosyl] ester (1), 3-O-ß-D-glucopyranosyl-(1→3)-[α-L-rhamnopyranosyl-(1→2)]-α-L-arabinopyranosyl-3ß,19α-dihydroxyoleanane-12-en-28-oic acid (2), lactifoloside C (3), lactifoloside H (4), randiasaponin IV (5), kudinoside G (6), ilexkudinoside W (7), lactifoloside G (8), kudinoside D (9), and ilexkudinoside T (10) by analyzing their HR-ESI-MS, NMR spectral data and by comparison with those reported in the literature. Compounds 1-10 were evaluated for anoctamin-1 (ANO1) inhibitory activity using yellow fluorescent protein reduction assays. At the concentration of 30 µM, compounds 2 and 9 displayed moderate ANO1 inhibitory percentages of 28.9 ± 0.85% and 26.2 ± 0.65%, respectively.

Analysis of volatile compound changes in fried shallot (Allium cepa L. var. aggregatum) oil at different frying temperatures by GC-MS, OAV, and multivariate analysis.

Flavor is a key attribute of fried oil that shows a critical correlation with temperature. Therefore, selecting the appropriate temperature is important in preparing fried shallot oil (FSO). Volatile compounds from five different FSOs were identified and comparatively studied using gas chromatography-mass spectrometry (GC-MS) coupled with multivariate data analysis, including principal component analysis (PCA) and orthogonal partial least squares discriminant analysis (OPLS-DA). GC-MS results identified a total of 93 volatiles, among which aldehydes, alcohols, pyrazines, and sulfur-containing compounds were the major compounds. Eighteen compounds had odor active values (OAV) >1. Among the compounds, hexanal, (E)-2-heptenal, (E)-2-octenal, dipropyl disulfide, 2-ethyl-3,5-dimethylpyrazine, and 1-octen-3-ol were important to the overall aroma profile of FSOs. In the PCA model, all the detected FSOs were divided into three clusters, which were assigned as cluster A (FSO5), B (FSO4), and C (the rest FSOs). Multivariate data analyses revealed that nonanal, 2-ethyl-5-methylpyrazine, (E,E)-2,4-decadienal, (E)-2-heptenal, and hexanal contributed positively to the classification of different FSOs. GC-MS coupled with multivariate data analysis could be used as a convenient and efficient analytical method to classify raw materials.

Cryopreservation of Plant Shoot Tips of Potato, Mint, Garlic, and Shallot Using Plant Vitrification Solution 3.

Cryopreservation of shoot tips facilitates long-term storage of plant genetic resources which can otherwise only be propagated vegetatively. The vitrification approach using the cryoprotectant plant vitrification solution 3 (PVS3, 50% sucrose and 50% glycerol) is easy to handle, has shown to produce high regrowth percentages in a number of potato, mint, garlic, and shallot accessions, and is, thus, highly suitable for routine cryopreservation of plant genetic resources. In the current chapter, the vitrification procedure is described for potato, mint, garlic, and shallot and includes details about modifications for the different plant species. Special emphasis is given on the preparation of the different culture media, solutions, the culture conditions prior and post-cryopreservation, and the preparation of the shoot tips from different sources. Furthermore, protocols to introduce plants into in vitro culture and methods to estimate cryopreservation success are provided.

Integration of Nitrogen and Biofertilizer on the Agronomic Efficiency and Quality of Shallot (Allium ascalonicum L.) Plant in the Rainy Season.

BACKGROUND AND OBJECTIVE: In the rainy season farmers don't interest to cultivate shallot because in addition to providing a high dosage of fertilizer they are also sensitive to pathogenic attacks so they are afraid of crop failure and cause low shallot production. This study aimed to knew effect of agronomic component and quality of shallot under different concentrations of biofertilizer and Ammonium Sulphate (AS) fertilizer dose in the rainy season. MATERIALS AND METHODS: The study was conducted in Cangkring, Srandakan, Bantul, Special Region of Yogyakarta Indonesia from August to October 2019. The study was arranged in RCBD factorial with three replications. The first factor was a various dose of ammonium sulphate (100, 200 and 300 kg ha-1). The second factor was various concentrations of biofertilizer (2, 3 and 4%), and control. The observed variables were the analysis of growth yield and quality component of shallot plant. The analyzed using analysis of variance at 5% of significance then continued by DMRT at 5% of significance. RESULTS: There was the interaction between the application of AS dosage and biofertilizer concentration on all of variable observations. There was a significant difference between treatment with control on all of the observation variables. CONCLUSION: The combination of AS fertilizer 200 kg ha-1 dose and 3% biofertilizer concentration increased agronomic efficiency, growth, bulbs yields, and quality of bulbs include provitamin A, oleoresin compounds.